Human Smad5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2250
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Smad5
Val155-Arg258
Accession # Q99717
Val155-Arg258
Accession # Q99717
Specificity
Detects human Smad5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Smad1, rhSmad2 and rhSmad4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Smad5 Antibody
Smad5 in Human Colon.
Smad5 was detected in immersion fixed paraffin-embedded sections of human colon using 5 µg/mL Human Smad5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2250) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Smad5 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon
Sample: Immersion fixed paraffin-embedded sections of human colon
Western Blot
0.1 µg/mL
Sample: Recombinant Human Smad5
Sample: Recombinant Human Smad5
Reviewed Applications
Read 2 reviews rated 4.5 using AF2250 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Smad5
Long Name
Mothers Against DPP Homolog 5
Alternate Names
Dwfc, JV5-1, MADH5
Entrez Gene IDs
4090 (Human)
Gene Symbol
SMAD5
UniProt
Additional Smad5 Products
Product Documents for Human Smad5 Antibody
Product Specific Notices for Human Smad5 Antibody
For research use only
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