Human SMOC-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5140
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry
Cited:
Functional Assay, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 667713
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human SMOC-2
Gln22-Gly446
Accession # Q9H3U7
Gln22-Gly446
Accession # Q9H3U7
Specificity
Detects human SMOC-2 in direct ELISAs and Western blots.
In Western blots, approximately 15%
cross-reactivity with recombinant human (rh) SMOC-1 and no cross-reactivity with
recombinant mouse SMOC-2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human SMOC-2 Antibody
SMOC-2 in Human Ovarian Cancer Tissue.
SMOC-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human SMOC-2 Monoclonal Antibody (Catalog # MAB5140) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human SMOC-2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SMOC-2
Long Name
Secreted Modular Calcium-binding Protein 2
Alternate Names
MST117, MSTP117, MSTP140, SMAP2, SMOC2
Gene Symbol
SMOC2
UniProt
Additional SMOC-2 Products
Product Documents for Human SMOC-2 Antibody
Product Specific Notices for Human SMOC-2 Antibody
For research use only
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