Human SNAP29 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7869
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 827003
Product Specifications
Immunogen
E. coli-derived recombinant human SNAP29
Met1-Glu129
Accession # O95721
Met1-Glu129
Accession # O95721
Specificity
Detects human SNAP29 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human SNAP29 Antibody
Detection of Human SNAP29 by Western Blot.
Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SNAP29 Monoclonal Antibody (Catalog # MAB7869) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SNAP29 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
SNAP29 in HepG2 Human Cell Line.
SNAP29 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human SNAP29 Monoclonal Antibody (Catalog # MAB7869) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human SNAP29 Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line
Western Blot
2 µg/mL
Sample: SK‑Mel‑28 human malignant melanoma cell line and K562 human chronic myelogenous leukemia cell line
Sample: SK‑Mel‑28 human malignant melanoma cell line and K562 human chronic myelogenous leukemia cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SNAP29
SNAP29 (29 kDa Soluble NSF [n-ethylmaleimide sensitive factor] Attachment Protein; also Synaptosomal associated protein 29 and GS32 [in rodent]) is a cytosolic and membrane-associated 27-32 kDa member of the syntaxin/ SNAP-25 family of proteins. It is widely, if not ubiquitously, expressed, and found in cell types as diverse as oligodentroglia, mast cells, neurons, Schwann cells and likely keratinocytes. Functionally, SNAP29 interacts with directly with syntaxin-1A and negatively impacts neurotransmission by inhibiting SNARE complex disassembly. In addition, it interacts with EHD1and AP-2, contributing to receptor-mediated endocytosis. Finally, it also might be said that SNAP29 is a key to the maintenance of general intracellular trafficking patterns. In this regard, SNAP29 has a remarkable ability to bind to a large number of syntaxins associated with multiple internal membranes. Human SNAP29 is 258 amino acids (aa) in length. It contains one t-SNARE coiled-coil homology domain (aa 196-258). Over aa 1-129, human and mouse SNAP29 share 88% aa sequence identity.
Long Name
Synaptosomal-associated Protein, 29 kDa
Alternate Names
CEDNIK
Gene Symbol
SNAP29
UniProt
Additional SNAP29 Products
Product Documents for Human SNAP29 Antibody
Product Specific Notices for Human SNAP29 Antibody
For research use only
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