Human SODD/BAG4 Antibody

Name: Human SODD/BAG4 Antibody
Brand: R&D Systems
SKU: AF4350
Rating: 3 (1 reviews)

R&D Systems, part of Bio-Techne | Catalog # AF4350

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunocytochemistry, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all SODD/BAG4 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SODD/BAG4
Glu122-Leu457
Accession # O95429

Specificity

Detects human SODD/BAG4 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human SODD/BAG4 Antibody

Detection of Human SODD/BAG4 by Western Blot.

Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line, Raji human Burkitt's lymphoma cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human SODD/BAG4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4350) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for SODD/BAG4 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

SODD/BAG4 in A431 Human Cell Line.

SODD/BAG4 was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human SODD/BAG4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4350) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot Shows Human SODD/BAG4 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SODD/BAG4 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human SODD/BAG4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4350) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SODD/BAG4 at approximately 72 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human SODD/BAG4 Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line

Knockout Validated

SODD/BAG4 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in SODD/BAG4 knockout HeLa cell line.

Western Blot

0.5 µg/mL
Sample: MOLT-4 human acute lymphoblastic leukemia cell line, Raji human Burkitt's lymphoma cell line, and A431 human epithelial carcinoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using AF4350 in the following applications:

ELISA (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SODD/BAG4

Tumor necrosis factor receptor-1 (TNF-R1) and other TNF receptor superfamily members, such as DR3, contain intracellular death domains (DD) and are capable of initiating apoptosis when activated by their ligands. Silencer of Death Domains (SODD) was identified as being involved in the cellular mechanism to protect against ligand-independent signaling by TNF-R1 and other DD receptors. SODD, also known as Bcl-2-Associated Athanogene 4 (BAG4), is a 457 amino acid (aa), anti‑apoptotic protein that functions through interactions with a variety of proteins including Bcl-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors, and members of the heat shock protein 70 kDa family. SODD is a ubiquitously expressed, cytoplasmic protein that contains a C‑terminal BAG domain that can bind and inhibit the chaperone activity of Hsc70/Hsp70. The association of SODD with the DD of TNF-R1 prevents constitutive activation of the TNF-R1 signaling pathway. Binding of TNF to TNF-R1 releases SODD and permits adapter molecules such as TRADD to associate with TNF-R1 leading to the activation of TNF signaling pathways such as apoptosis and NF kappaB activation.

Long Name

Silencer of Death Domains

Alternate Names

BAG4

Entrez Gene IDs

9530 (Human); 67384 (Mouse); 361167 (Rat)

Gene Symbol