Human SorLA Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5699
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 525122
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human SorLA
Ser82-Ser1550
Accession # Q92673
Ser82-Ser1550
Accession # Q92673
Specificity
Detects human SorLA in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human SorLA Antibody
SorLA in Human Brain.
SorLA was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human SorLA Monoclonal Antibody (Catalog # MAB5699) at 25 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific labeling was localized to the cytoplasmic granules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
SorLA in Human Brain.
SorLA was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human SorLA Monoclonal Antibody (Catalog # MAB5699) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human SorLA Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SorLA
Long Name
Sortilin-related Receptor Containing LDLR Class A Repeats
Alternate Names
gp250, LR11, LRP9, SORL1
Gene Symbol
SORL1
UniProt
Additional SorLA Products
Product Documents for Human SorLA Antibody
Product Specific Notices for Human SorLA Antibody
For research use only
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