Human SOX10 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB28643
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1017727
Product Specifications
Immunogen
Synthetic human SOX10 peptide
Accession # P56693
Accession # P56693
Specificity
Detects human SOX10 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human SOX10 Antibody
Detection of Human SOX10 by Western Blot.
Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SOX10 Monoclonal Antibody (Catalog # MAB28643) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SOX10 at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
SOX10 in SK‑Mel‑28 Human Cell Line.
SOX10 was detected in immersion fixed SK-Mel-28 human malignant melanoma cell line (positive stain) and U2OS human osteosarcoma cell line (negative stain) using Mouse Anti-Human SOX10 Monoclonal Antibody (Catalog # MAB28643) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human SOX10 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed SK‑Mel‑28 human malignant melanoma cell line
Sample: Immersion fixed SK‑Mel‑28 human malignant melanoma cell line
Western Blot
2 µg/mL
Sample: SK‑Mel‑28 human malignant melanoma cell line
Sample: SK‑Mel‑28 human malignant melanoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX10
References
- Lo, L. et al. (2002) Development 129:1553.
Long Name
Transcription Factor SOX10
Alternate Names
DOM, WS4
Entrez Gene IDs
6663 (Human)
Gene Symbol
SOX10
UniProt
Additional SOX10 Products
Product Documents for Human SOX10 Antibody
Product Specific Notices for Human SOX10 Antibody
For research use only
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