Human SRPK3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB72301
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 722516
Product Summary for Human SRPK3 Antibody
Immunogen
E. coli-derived recombinant human SRPK3
Arg247-Ser316
Accession # Q9UPE1
Arg247-Ser316
Accession # Q9UPE1
Specificity
Detects human SRPK3 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human SRPK3 Antibody
Detection of Human SRPK3 by Western Blot.
Western blot shows lysates of human skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SRPK3 Monoclonal Antibody (Catalog # MAB72301) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SRPK3 at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
SRPK3 in Human Skeletal Muscle.
SRPK3 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Human SRPK3 Monoclonal Antibody (Catalog # MAB72301) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human SRPK3 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skeletal muscle
Sample: Immersion fixed paraffin-embedded sections of human skeletal muscle
Western Blot
1 µg/mL
Sample: Human skeletal muscle tissue
Sample: Human skeletal muscle tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SRPK3
Long Name
Ser/Arg-rich Protein-specific Kinase 3
Alternate Names
MSSK1, STK23
Gene Symbol
SRPK3
UniProt
Additional SRPK3 Products
Product Documents for Human SRPK3 Antibody
Product Specific Notices for Human SRPK3 Antibody
For research use only
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