Human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6905
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1
Accession # Q11201
Accession # Q11201
Specificity
Detects human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human ST3GAL2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Antibody
Detection of Human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 by Western Blot.
Western blot shows lysates of human placenta. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ST3 beta-Gal a-2,3-Sialyltransferase 1/ST3GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6905) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST3 beta-Gal a-2,3-Sialyltransferase 1/ ST3GAL1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human placenta
Sample: Human placenta
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1
ST3 beta-Gal alpha-2,3-Sialyltransferase 1 (ST3GAL1, also known as SIAT4 and SIAT4A) is a 45 kDa member of glycosyltransferase family 29 that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The full length ST3GAL1is a type II Golgi membrane glycoprotein that can be proteolytically processed to a soluble form. ST3Gal1 contributes to the alpha2-3-sialylation of O-glycans such as T antigens. Upon maturation of Dendritic cells derived from monocytes ST3GAl1 is altered in a stimulus-dependent manner. Multiple isoforms of human ST3GAL1 have been described. Human ST3GAL1 shares 85% identity with mouse ST3GAL1.
Long Name
ST3 beta-Galactoside alpha-2,3-Sialyltransferase 1
Alternate Names
Gal-NAc6S, SIAT4A, SIATFL, ST3GalA, ST3O
Gene Symbol
ST3GAL1
UniProt
Additional ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Products
Product Documents for Human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Antibody
Product Specific Notices for Human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Antibody
For research use only
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