Human ST6 GalNAc alpha-2,6-sialyltransferase V/ST6GALNAC5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB67151
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 719508
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human SGalNAc alpha-2,6-sialyltransferase V/ST6GALNAC5
Gly30-Phe336
Accession # Q9BVH7
Gly30-Phe336
Accession # Q9BVH7
Specificity
Detects human GalNAc alpha-2,6-sialyltransferase V/ST6GALNAC5 in direct ELISAs. In direct ELISAs, no cross-reactivity
with recombinant human (rh) ST6GALNAC4 or rhST6GALNAC6 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human ST6 GalNAc alpha-2,6-sialyltransferase V/ST6GALNAC5 Antibody
GalNAc alpha‑2,6‑sialyltransferase V/ST6GALNAC5 in MDA‑MB‑231 Human Cell Line.
GalNAc a-2,6-sialyltransferase V/ST6GALNAC5 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Mouse Anti-Human GalNAc a-2,6-sialyltransferase V/ST6GALNAC5 Monoclonal Antibody (Catalog # MAB67151) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human ST6 GalNAc alpha-2,6-sialyltransferase V/ST6GALNAC5 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MDA‑MB‑231 human breast cancer cell line
Sample: Immersion fixed MDA‑MB‑231 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ST6 GalNAc alpha-2,6-sialyltransferaseV/ST6GALNAC5
References
- Kolter, T. et al. (2002) J. Biol. Chem. 277:25859.
- Yu, R.K. et al. (2008) Glycoscience DOI: 10.1007/978-3-540-30429-6_41.
- Okajima, T. et al. (1999) J. Biol. Chem.274:30557.
- Harduin-Lepers, A. et al. (2005) Glycobiology 15:805.
- Bos, P.D. et al. (2009) Nature 459:1005.
- Arshad, F. et al. (2011). Patholog. Res. Int. DOI: 10.4061/2011/920509.
- Wu, Z.L. et al. (2010) Glycobiology DOI: 10.1093/glycob/cwq187.
Long Name
ST6 alpha-N-acetylGalactosaminide alpha-2,6-sialyltransferase 5
Alternate Names
GD1 alpha synthase, SIAT7E, ST6GalNAcV
Gene Symbol
ST6GALNAC5
UniProt
Additional ST6 GalNAc alpha-2,6-sialyltransferaseV/ST6GALNAC5 Products
Product Documents for Human ST6 GalNAc alpha-2,6-sialyltransferase V/ST6GALNAC5 Antibody
Product Specific Notices for Human ST6 GalNAc alpha-2,6-sialyltransferase V/ST6GALNAC5 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...