Human STAT6 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2167
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Immunocytochemistry, Intracellular Staining by Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 253906
Product Specifications
Immunogen
E. coli-derived recombinant human STAT6
Asn341-Gly640
Accession # P42226.1
Asn341-Gly640
Accession # P42226.1
Specificity
Detects human, mouse, and rat STAT6.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human STAT6 Antibody
STAT6 in Human PBMCs.
STAT6 was detected in immersion fixed PHA-stimulated human peripheral blood mononuclear cells (PBMCs) using 8 µg/mL Mouse Anti-Human STAT6 Monoclonal Antibody (Catalog # MAB2167) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Detection of Human STAT6 by Western Blot.
Western Blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human STAT6 Monoclonal Antibody (Catalog # MAB2167) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for STAT6 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human STAT6 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed PHA-stimulated human peripheral blood mononuclear cells (PBMCs)
Sample: Immersion fixed PHA-stimulated human peripheral blood mononuclear cells (PBMCs)
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with methanol
Sample: Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with methanol
Western Blot
2 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line
Sample: Daudi human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STAT6
Long Name
Signal Transducer and Activator of Transcription 6
Alternate Names
D12S1644, EC 2.4.1.227, EC 2.7.7.6, IL-4 Stat, IL-4-STAT, signal transducer and activator of transcription 6, signal transducer and activator of transcription 6, interleukin-4 induced, STAT, interleukin-4 induced, STAT, interleukin4-induced, STAT6B, STAT6C, transcription factor IL-4 STAT
Gene Symbol
STAT6
UniProt
Additional STAT6 Products
Product Documents for Human STAT6 Antibody
Product Specific Notices for Human STAT6 Antibody
For research use only
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