Human STEAP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5587
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 1057323
Product Summary for Human STEAP1 Antibody
Immunogen
E. coli-derived recombinant human STEAP-1
Met1-Trp71
Accession # Q9UHE8
Met1-Trp71
Accession # Q9UHE8
Specificity
Detects human STEAP-1 in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human STEAP1 Antibody
Detection of Human STEAP1 by Western Blot.
Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human STEAP1 Monoclonal Antibody (Catalog # MAB5587) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for STEAP1 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of STEAP1 in HEK293 transfected with Human STEAP1 and eGFP cells by Flow Cytometry.
HEK293 transfected with Human STEAP1 and eGFP were stained with eGFP and either (A) Mouse Anti-Human STEAP1 Monoclonal Antibody (Catalog # MAB5587) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.Applications for Human STEAP1 Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 transfected with Human STEAP1 and eGFP
Sample: HEK293 transfected with Human STEAP1 and eGFP
Western Blot
2 µg/mL
Sample: LNCaP human prostate cancer cell line
Sample: LNCaP human prostate cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STEAP1
Long Name
Six Transmembrane Epithelial Antigen of the Prostate 1
Alternate Names
PRSS24
Gene Symbol
STEAP1
UniProt
Additional STEAP1 Products
Product Documents for Human STEAP1 Antibody
Product Specific Notices for Human STEAP1 Antibody
For research use only
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