Human TACI/TNFRSF13B Antibody
R&D Systems, part of Bio-Techne | Catalog # AF174
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Blockade of Receptor-ligand Interaction, Immunohistochemistry, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TACI/TNFRSF13B
Ser2-Thr166
Accession # Q53F36
Ser2-Thr166
Accession # Q53F36
Specificity
Detects human TACI in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse TACI and recombinant human BCMA is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human TACI/TNFRSF13B Antibody
TACI/TNFRSF13B in Human Spleen.
TACI/TNFRSF13B was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human TACI/TNFRSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF174) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific labeling was localized to lymphocytes in the marginal zone. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human TACI/TNFRSF13B Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.075-0.3 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) to immobilized Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human spleen subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Western Blot
0.1 µg/mL
Sample: Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC)
Sample: Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TACI/TNFRSF13B
N‑terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine rich domains. Within the TNFRSF, it shares the highest homology with BCMA. TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Human TACI is a 293 amino acid (aa) protein consisting of a 166 aa extracellular domain, a 20 aa transmembrane domain, and a 107 aa intracellular domain. Human and mouse TACI share 54% amino acid identity.
References
- Xia, X-Z. et al. (2000) J. Exp. Med. 192:137.
- von Bulow, G.U. et al. (1997) Science 278:138.
- Gross, J.A. et al. (2000) Nature 404:995.
- Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
- Yan, M. et al. (2000) Nature Immunol. 1:37.
- Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.
Long Name
Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B
Alternate Names
CD267, CVID, TNFRSF13B
Gene Symbol
TNFRSF13B
UniProt
Additional TACI/TNFRSF13B Products
Product Documents for Human TACI/TNFRSF13B Antibody
Product Specific Notices for Human TACI/TNFRSF13B Antibody
For research use only
Loading...
Loading...
Loading...
Loading...