HEK293 cells transfected with Human TAPBL and eGFP vs irrelevant were stained with eGFP and Mouse Anti-Human TAPBPR Monoclonal Antibody (A, B) (Catalog # MAB11337) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TAPBPR Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 cells transfected with Human TAPBL and eGFP vs irrelevant

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TAPBPR

TAP-binding protein-like (TAPBPL), also known as TAP binding protein-related (TAPBPR) and Tapasin-related protein (TAPASINR) is a transmembrane protein of the Immunoglobulin (Ig) superfamily (1, 2). TAPBPR was originally isolated as a homologue to TAPASIN but more recently was identified as a novel B7 family-related molecule since it shares sequence, structural, and functional similarities to B7 family members (3). Mature human TAPBPR consists of a lumenal domain containing an IgV and IgC domain, a transmembrane domain, and a cytoplasmic tail which lacks an ER retention motif. Within the lumenal domain, mature human TAPBPR shares 70% and 71% amino acid sequence identity with mouse and rat TAPBPR, respectively. Multiple alternatively spliced TAPBPR isoforms are known to exist with unique properties (4).TAPBPR is widely expressed and, similar to TAPASIN, functions as a both a chaperone protein and peptide editor of MHC class I, but in a peptide‑loading complex (PLC) independent manner (5, 6). TAPBPR decreases the rate at which MHC class I molecules mature through the secretory pathway, a role which could be important for peptide selection by MHC class I molecules (7). TAPBPR is also expressed on the surface of T cells and antigen-presenting cells (APCs) and plays an inhibitory role in the proliferation and activation of T cells (4). TAPBPR can be expressed on various cancer cells like leukemia and has the potential to be used in the treatment of autoimmune diseases and transplant rejection, as well as cancer (4).

References

Hermann, C. et al. (2015) Tissue antigens 85(3):155.
Teng, M. et al. (2002) European Journal of Immunology 32:1059.
Lin, Y. et al. (2021). EMBO Mol Med. 13(5):13404.
Porter, K.M. et al. (2014) Immunology 142:289.
Margulies, D. et al. (2020) Current Opinion in Immunology 64:71.
Boyle, L.H. et al. (2013) PNAS 110:3465.
Hermann, C. et al. (2013) Journal of Immunology 191(11):5743.

Long Name

Tapasin Related Protein

Alternate Names

Tapasin-like, TAPBPL, TAPBPR

Entrez Gene IDs

55080 (Human); 213233 (Mouse); 297602 (Rat)

Gene Symbol

TAPBPL

UniProt

Q9BX59.2

Additional TAPBPR Products

Product Documents for Human TAPBPR Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TAPBPR Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human TAPBPR Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11337

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all TAPBPR Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human TAPBPR Antibody

Detection of TAPBPR in HEK293 cell transfected with Human TAPBL and eGFP vs irrelevant cells by Flow Cytometry.