Human Tau Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10629
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1032714
Product Specifications
Immunogen
Human Tau H synthetic peptide
Accession # P10636
Accession # P10636
Specificity
Detects Human Tau in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Tau Antibody
Tau in Human Brain (Alzheimer's).
Tau was detected in immersion fixed paraffin-embedded sections of human brain (Alzheimer's) using Mouse Anti-Human Tau Monoclonal Antibody (Catalog # MAB10629) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Tau Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (Alzheimer's)
Sample: Immersion fixed paraffin-embedded sections of human brain (Alzheimer's)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tau
Tau protein is named for its ability to serve as a bridge for microtubule formation and stabilization. Tau in PNS neurons is 758 amino acids (aa) in length and 110 kDa in size. It migrates anomalously in SDS-PAGE. Its N-terminus binds plasma membrane components while its C‑terminus binds microtubules. The microtubule-binding region contains four consecutive Tau/MAP repeats between aa 561‑685. Each repeat is 30 aa long, Ser/Thr rich, and shows a PGGG motif. Multiple isoforms exist, ranging from 316 to 776 aa in length. Various deletions occur within the first 500 aa, with some isoforms also showing an absence of the second Tau repeat (aa 592‑622). Between aa 624‑756, human Tau protein shows absolute identity to mouse and dog Tau.
Long Name
Microtubule-Associated Protein Tau
Alternate Names
MAPT, MSTD, MTBT1, Neurofibrillary tangle protein, PPND
Gene Symbol
MAPT
UniProt
Additional Tau Products
Product Documents for Human Tau Antibody
Product Specific Notices for Human Tau Antibody
For research use only
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