Human Tau MAb (Clone 376720)
R&D Systems, part of Bio-Techne | Catalog # MAB3494
Key Product Details
Species Reactivity
Multi-Species
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 376720
Product Specifications
Immunogen
E. coli-derived recombinant human Tau
Gln624-Gln756
Accession # P10636
Gln624-Gln756
Accession # P10636
Specificity
Detects human Tau in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Tau MAb (Clone 376720)
Tau in Human Brain.
Tau was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Tau Monoclonal Antibody (Catalog # MAB3494) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Tau MAb (Clone 376720)
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's brain
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's brain
Western Blot
1 µg/mL
Sample: Recombinant Human Tau
Sample: Recombinant Human Tau
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB3494 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tau
Tau protein is named for its ability to serve as a bridge for microtubule formation and stabilization. Tau in PNS neurons is 758 amino acids (aa) in length and 110 kDa in size. It migrates anomalously in SDS-PAGE. Its N-terminus binds plasma membrane components while its C‑terminus binds microtubules. The microtubule-binding region contains four consecutive Tau/MAP repeats between aa 561‑685. Each repeat is 30 aa long, Ser/Thr rich, and shows a PGGG motif. Multiple isoforms exist, ranging from 316 to 776 aa in length. Various deletions occur within the first 500 aa, with some isoforms also showing an absence of the second Tau repeat (aa 592‑622). Between aa 624‑756, human Tau protein shows absolute identity to mouse and dog Tau.
Long Name
Microtubule-Associated Protein Tau
Alternate Names
MAPT, MSTD, MTBT1, Neurofibrillary tangle protein, PPND
Gene Symbol
MAPT
UniProt
Additional Tau Products
Product Documents for Human Tau MAb (Clone 376720)
Product Specific Notices for Human Tau MAb (Clone 376720)
For research use only
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