Human TCF-12/HTF4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB69281

R&D Systems, part of Bio-Techne
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MAB69281
MAB69281-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 690129

View all TCF-12/HTF4 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TCF-12/HTF4
Met108-Glu521
Accession # Q99081

Specificity

Detects human TCF-12/HTF4 in direct ELISAs. In direct ELISAs, 75%‑100% cross-reactivity with recombinant human (rh) TCF-3 and no cross-reactivity with rhTCF-1, -2, -4, or rhStathmin-2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TCF-12/HTF4 Antibody

TCF-12/HTF4 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

TCF-12/HTF4 in HeLa Human Cell Line.

TCF-12/HTF4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human TCF-12/HTF4 Monoclonal Antibody (Catalog # MAB69281) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human TCF-12/HTF4 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line 

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TCF-12/HTF4

TCF-12 (transcription factor 12), also called HTF4, bHLHb20 and HEB, is a 682 amino acid (aa) DNA binding protein of the E protein family of helix-loop-helix (HLH) transcription factors. E proteins, including TCF-12 and E2A, mediate lymphocyte differentiation and also function in neurogenesis. Homo-oligomerization is required for activity; hetero-oligomers are also formed. The region of human TCF-12 used as an immunogen excludes the HLH domain and shares 94% aa sequence identity with mouse and rat TCF-12. Potential isoforms of 706 and 648 aa contain an insertion of 24 aa after aa 396, while isoforms of 648, 624 and 512 aa show alternate N‑terminal sequences that merge with the 682 aa form at aa 75, 75 and 194, respectively.

Long Name

Transcription Factor 12

Alternate Names

BHLHB20, HEB, HTF4, TCF12

Entrez Gene IDs

6938 (Human); 21406 (Mouse); 25720 (Rat)

Gene Symbol

TCF12

UniProt

Q99081

Additional TCF-12/HTF4 Products

Product Documents for Human TCF-12/HTF4 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TCF-12/HTF4 Antibody

For research use only

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