Human TDAG8/GPR65 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10077
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 976128
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human TDAG8/GPR65
Met1-Glu337
Accession # Q8IYL9
Met1-Glu337
Accession # Q8IYL9
Specificity
Detects human TDAG8/GPR65 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human TDAG8/GPR65 Antibody
Detection of Human TDAG8/GPR65 by Western Blot.
Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human TDAG8/GPR65. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TDAG8/GPR65 Monoclonal Antibody (Catalog # MAB10077) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for TDAG8/GPR65 at approximately 85-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TDAG8/GPR65 in Transfected HEK293 Human Cell Line.
TDAG8/GPR65 was detected in immersion fixed TDAG8/GPR65-transfected HEK293 human embryonic kidney cell line (left panel, positive stain) and non-transfected HEK293 human embryonic kidney cell line (right panel, negative stain) using Mouse Anti-Human TDAG8/GPR65 Monoclonal Antibody (Catalog # MAB10077) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human TDAG8/GPR65 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed TDAG8/GPR65-transfected HEK293 human embryonic kidney cell line
Sample: Immersion fixed TDAG8/GPR65-transfected HEK293 human embryonic kidney cell line
Western Blot
2 µg/mL
Sample: NS0 mouse myeloma cell line transfected with human TDAG8/GPR65
Sample: NS0 mouse myeloma cell line transfected with human TDAG8/GPR65
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TDAG8/GPR65
Long Name
G Protein-coupled Receptor 65
Alternate Names
GPR65, Psychosine Receptor
Gene Symbol
GPR65
UniProt
Additional TDAG8/GPR65 Products
Product Documents for Human TDAG8/GPR65 Antibody
Product Specific Notices for Human TDAG8/GPR65 Antibody
For research use only
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