Human TFEB Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9170
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 954604
Product Specifications
Immunogen
Human TFEB synthetic peptide
Pro384-Ala446
Accession # P19484
Pro384-Ala446
Accession # P19484
Specificity
Detects human TFEB in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human TFEB Antibody
Detection of Human TFEB by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TFEB Monoclonal Antibody (Catalog # MAB9170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TFEB at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TFEB in A549 Human Cell Line.
TFEB was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human TFEB Monoclonal Antibody (Catalog # MAB9170) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human TFEB Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line
Sample: Immersion fixed A549 human lung carcinoma cell line
Western Blot
2 µg/mL
Sample: Raji human Burkitt's lymphoma cell line
Sample: Raji human Burkitt's lymphoma cell line
Reviewed Applications
Read 1 review rated 5 using MAB9170 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TFEB
Long Name
Transcription Factor EB
Alternate Names
ALPHATFEB, BHLHE35, TCFEB
Gene Symbol
TFEB
UniProt
Additional TFEB Products
Product Documents for Human TFEB Antibody
Product Specific Notices for Human TFEB Antibody
For research use only
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