Human TFF2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4077
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 366508
Product Specifications
Immunogen
E. coli-derived recombinant human TFF2
Glu24-Tyr129
Accession # Q03403
Glu24-Tyr129
Accession # Q03403
Specificity
Detects human TFF2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human TFF3.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human TFF2 Antibody
TFF2 in Human Stomach.
TFF2 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human TFF2 Monoclonal Antibody (Catalog # MAB4077) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human TFF2 Antibody
Application
Recommended Usage
Immunohistochemistry
1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach
Sample: Immersion fixed paraffin-embedded sections of human stomach
Western Blot
1 µg/mL
Sample: Recombinant Human TFF2
Sample: Recombinant Human TFF2
Reviewed Applications
Read 1 review rated 5 using MAB4077 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TFF2
Trefoil Factor 2 (TFF2), previously called spasmolysin, is one of three trefoil peptides secreted by epithelial cells that line mucus membranes. Trefoils contribute to protection and repair of the gastrointestinal tract. TFF2 is secreted by epithelia of the basal gastric glands and duodenal Brunner’s glands, but is also found in spleen, thymus and lung and may modulate leukocyte recruitment to sites of inflammation in these tissues. The 129 amino acid (aa) human TFF2 contains two trefoil structures formed by intramolecular disulfides and shows 82% aa identity with mouse TFF2.
Long Name
Trefoil Factor 2
Alternate Names
SML1, SP, Spasmolysin, Spasmolytic Polypeptide
Gene Symbol
TFF2
UniProt
Additional TFF2 Products
Product Documents for Human TFF2 Antibody
Product Specific Notices for Human TFF2 Antibody
For research use only
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