Human TFF2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4077
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human TFF2
Glu24-Tyr129
Accession # Q03403
Glu24-Tyr129
Accession # Q03403
Specificity
Detects human TFF2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human TFF3 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human TFF2 Antibody
Detection of Human TFF2 by Western Blot.
Western blot shows lysates of human stomach tissue, human stomach cancer tissue, human breast tissue, and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TFF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4077) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TFF2 at approximately 19-21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human TFF2 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample:
Sample:
Human stomach tissue, human stomach cancer tissue, human breast tissue, and human kidney tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TFF2
Trefoil factor 2 (TFF2), also known as spasmolytic polypeptide (Sp) is a 20 kDa secreted trefoil factor family protein. It is a protease-resistant glycoprotein primarily produced by mucous-secreting cells of the stomach and duodenum and is upregulated in response to inflammation. TFF2 interacts with mucin and other TFF2-binding proteins to protect and repair mucosal epithelium. TFF2 is also expressed by macrophages and lymphocytes and may have a role in modulating immune system responses. The 106 residue mature human TFF2 contains two trefoil domains (aa 36-72 and aa 80-121) and one N-linked glycosylation site. Human TFF2 shares 71% and 87% aa sequence identity with canine and mouse TFF2, respectively.
Long Name
Trefoil Factor 2
Alternate Names
SML1, SP, Spasmolysin, Spasmolytic Polypeptide
Gene Symbol
TFF2
UniProt
Additional TFF2 Products
Product Documents for Human TFF2 Antibody
Product Specific Notices for Human TFF2 Antibody
For research use only
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