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Human TFF3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4408

R&D Systems, part of Bio-Techne
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MAB4408
MAB4408-SP

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 415921

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TFF3
Ala21-Phe80
Accession # Q07654

Specificity

Detects human TFF3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human TFF2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human TFF3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human TFF3

Reviewed Applications

Read 4 reviews rated 3.8 using MAB4408 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFF3

Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes. Trefoils contribute to protection and repair of the gastrointestinal tract. TFF3 is secreted by mucus-producing goblet cells in the large and small intestines and lower respiratory tract. It is mainly active as a disulfide-linked homodimer. The mature 59 amino acid (aa), 6.5 kDa human TFF3 contains one trefoil structure formed by intramolecular disulfides and shows 76% aa identity with mouse TFF3.

Long Name

Trefoil Factor 3

Alternate Names

ITF, TFI

Entrez Gene IDs

7033 (Human); 21786 (Mouse); 25563 (Rat)

Gene Symbol

TFF3

UniProt

Additional TFF3 Products

Product Documents for Human TFF3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TFF3 Antibody

For research use only

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