Human TfR2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3120
Key Product Details
Species Reactivity
Human
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TfR2
Arg105-Phe801
Accession # Q9UP52
Arg105-Phe801
Accession # Q9UP52
Specificity
Detects human TfR2 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human TfR2 Antibody
Detection of Human TfR2 by Western Blot.
Western blot shows lysates of human liver tissue and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TfR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3120) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for TfR2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human TfR2 by Simple WesternTM.
Simple Western lane view shows lysates of human liver tissue and K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for TfR2 at approximately 50 kDa (as indicated) using 50 µg/mL of Goat Anti-Human TfR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3120) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human TfR2 Antibody
Application
Recommended Usage
Simple Western
50 µg/mL
Sample: Human liver tissue and K562 human chronic myelogenous leukemia cell line
Sample: Human liver tissue and K562 human chronic myelogenous leukemia cell line
Western Blot
2 µg/mL
Sample: Human liver tissue and K562 human chronic myelogenous leukemia cell line
Sample: Human liver tissue and K562 human chronic myelogenous leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TfR2
Long Name
Transferrin Receptor 2
Alternate Names
HFE3, TFRC2
Gene Symbol
TFR2
UniProt
Additional TfR2 Products
Product Documents for Human TfR2 Antibody
Product Specific Notices for Human TfR2 Antibody
For research use only
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