Species Reactivity:

Human

Immunogen:

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Thrombopoietin/Tpo
Ser22-Gly353
Accession # P40225

Applications:

Western Blot

Label:

Unconjugated

Source:

Monoclonal Mouse IgG₁ Clone # 1065103

Specificity:

Detects recombinant protein specific for human Thrombopoietin in Direct ELISA.

View Full Technical Details for Human Thrombopoietin/Tpo Antibody

Technical Data
Citations / Reviews

Applications for Human Thrombopoietin/Tpo Antibody

	Recommended Concentration	Sample
Western Blot	2 µg/mL	Human liver tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Thrombopoietin/Tpo Antibody

	Western Blot Detection of Human Thrombopoietin/Tpo by Western Blot. Western Blot shows lysates of human liver tissue. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human Thrombopoietin/Tpo Monoclonal Antibody (Catalog # MAB11609) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Thrombopoietin/Tpo at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Formulation, Preparation and Storage

Purification	Protein A or G purified from ascites
Reconstitution	Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thrombopoietin/Tpo

Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders (1-3). The 353 amino acid (aa) human Tpo precursor is cleaved to yield the 332 aa mature protein. Mature human Tpo shares approximately 70% aa sequence homology with mouse and rat Tpo. It is an 80-85 kDa protein that consists of an N-terminal domain with homology to Erythropoietin (Epo) and a C-terminal domain that contains multiple N-linked and O-linked glycosylation sites (4, 5). Tissue specific alternate splicing of human Tpo generates multiple isoforms with internal deletions, insertions, and/or C-terminal substitutions (6). Tpo promotes the differentiation, proliferation, and maturation of megakaryocytes (MK) and their progenitors (4, 5, 7). Several other cytokines can promote these functions as well but only in cooperation with Tpo (8, 9). Notably, IL-3 independently induces MK development, although its effects are restricted to early in the MK lineage (8, 9). Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation (10 - 13). It is cleaved by platelet-derived thrombin following Arg191 within the C-terminal domain and subsequently at other sites upon extended digestion (14). Full length Tpo and shorter forms circulate in the plasma (4, 5). The C terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation (5). Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling (15, 16).

Item	Value
References	Deutsch, V.R. and A. Tomer (2006) Br. J. Haematol. 134:453. Kaushansky, K. (2005) J. Clin. Invest. 115:3339. Li, J. et al. (1999) Br. J. Haematol. 106:345. Bartley, T.D. et al. (1994) Cell 77:1117. de Sauvage, F.J. et al. (1994) Nature 369:533. Marcucci, R. and M. Romano (2008) Biochim. Biophys. Acta 1782:427. Kaushansky, K. et al. (1994) Nature 369:568. Kaushansky, K. et al. (1995) Proc. Natl. Acad. Sci. 92:3234. Broudy, V.C. et al. (1995) Blood 85:1719. Lok, S.I. et al. (1994) Nature 369:565. Chen, J. et al. (1995) Blood 86:4054. Oda, A. et al. (1996) Blood 87:4664. Van Os, E. et al. (2003) Br. J. Haematol. 121:482. Kato, T. et al. (1997) Proc. Natl. Acad. Sci. 94:4669. Ehrenreich, H. et al. (2005) Proc. Natl. Acad. Sci. 102:862. Samoylenko, A. et al. (2008) Cell. Signal. 20:154.
Entrez Gene IDs	7066 (Human); 21832 (Mouse); 81811 (Rat)
Alternate Names	MGDF, MGDFC-mpl ligand, MK-CSF, MKCSF, ML, MPL ligand, MPLLG, MPLLGMGC163194, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, THCYT1, THPO, TPOMKCSF, Thrombopoietin, Tpo, megakaryocyte stimulating factor, thrombopoietin nirs variant 1

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Human Thrombopoietin/Tpo Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11609

Key Product Details

Applications for Human Thrombopoietin/Tpo Antibody

Scientific Data Examples for Human Thrombopoietin/Tpo Antibody

Western Blot

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Thrombopoietin/Tpo

Customer Reviews for Human Thrombopoietin/Tpo Antibody

Product Documents for Human Thrombopoietin/Tpo Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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