Human TIM-3 Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2365G

Clone 344823 was used by HLDA to establish CD designation
R&D Systems, part of Bio-Techne
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FAB2365G
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 344823

View all TIM-3 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TIM-3
Ser22-Arg200
Accession # Q8TDQ0.2

Specificity

Detects human TIM‑3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM‑1, rhTIM‑4, recombinant mouse (rm) TIM‑1, rmTIM‑2, rmTIM‑3, rmTIM‑5, or rmTIM‑6.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human TIM-3 Alexa Fluor® 488-conjugated Antibody

Detection of TIM-3 antibody in Human PBMCs Monocytes antibody by Flow Cytometry.

Detection of TIM‑3 in Human PBMCs Monocytes by Flow Cytometry.

Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Rat Anti-Human TIM-3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB2365G, filled histogram) or isotype control antibody (Catalog # IC006G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of TIM-3 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of TIM‑3 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with Recombinant Human IL-12 (Catalog # 219-IL) and Goat Anti-Human IL-4 Polyclonal Antibody (Catalog # AB-204-NA) to induce Th1 development for 3 days were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Rat Anti-Human TIM-3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB2365G) or (B) Rat IgG2AAlexa Fluor 488 Isotype Control (Catalog # IC006G). Dot plots were gated on lymphocytes. View our protocol for Staining Membrane-associated Proteins.

Applications for Human TIM-3 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: Human peripheral blood mononuclear cell (PBMC) monocytes (untreated) and Human peripheral blood mononuclear cells (PBMCs) treated with Recombinant Human IL‑12 (Catalog # 219-IL) and Goat Anti-Human IL‑4 Polyclonal Antibody (Catalog # AB-204-NA) to induce Th1 development
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TIM-3

TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region (1, 2). Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (3). An alternatively spliced isoform is truncated within the mucin-like stalk. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg) (3-10). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity (11). TIM-3 ligation attenuates CD8+ and Th1 cell responses (11-13) and promotes the activity of Treg and myeloid derived suppressor cells (8, 11, 13, 14). In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens (4). It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA (7). TIM-3 interactions with Galectin-9 can alternatively trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity (10).

References

  1. Sakuishi, K. et al. (2011) Trends Immunol. 32:345.
  2. Anderson, A.C. (2012) Curr. Opin. Immunol. 24:213.
  3. Monney, L. et al. (2002) Nature 415:536.
  4. Nakayama, M. et al. (2009) Blood 113:3821.
  5. Anderson, A.C. et al. (2007) Science 318:1141.
  6. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
  7. Chiba, S. et al. (2012) Nat. Immunol. 13:832.
  8. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
  9. Ndhlovu, L.C. et al. (2012) Blood 119:3734.
  10. Gleason, M.K. et al. (2012) Blood 119:3064.
  11. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  12. Sakhdari, A. et al. (2012) PLoS ONE 7:e40146.
  13. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
  14. Dardalhon, V. et al. (2010) J. Immunol. 185:1383.

Long Name

T Cell Immunoglobulin Mucin-3

Alternate Names

CD366, HAVcr-2, HAVCR2, KIM-3, SPTCL, TIM3, TIMD3

Entrez Gene IDs

84868 (Human); 171285 (Mouse); 363578 (Rat); 479318 (Canine); 102141722 (Cynomolgus Monkey)

Gene Symbol

HAVCR2

UniProt

Q8TDQ0.2

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Certificate of Analysis

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Product Specific Notices for Human TIM-3 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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