Human TMEM119 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB103131
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2699A
Product Specifications
Immunogen
Chinese Hamster Ovary cell line CHO derived human TREM119
Arg26-Met96
Accession # Q4V9L6.1
Arg26-Met96
Accession # Q4V9L6.1
Specificity
Detects human TREM119 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human TMEM119 Antibody
TMEM119 in Human lymph node.
TMEM119 was detected in immersion fixed paraffin-embedded sections of human lymph node using Rabbit Anti-Human TMEM119 Monoclonal Antibody (Catalog # MAB103131) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane and cytoplasm in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of TMEM119 in HEK293 Human Cell Line Transfected with Human TMEM119 and eGFP by Flow Cytometry
HEK293 human embryonic kidney cell line transfected with (A) human TMEM119 or (B) irrelevant protein, and eGFP was stained with Rabbit Anti-Human TMEM119 Monoclonal Antibody (Catalog # MAB103131) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Quadrant markers were set based on Rabbit IgG Isotype Control (MAB1050, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.Applications for Human TMEM119 Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human TMEM119 and eGFP
Sample: HEK293 Human Cell Line Transfected with Human TMEM119 and eGFP
Immunohistochemistry
1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymph node
Sample: Immersion fixed paraffin-embedded sections of human lymph node
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TMEM119
TMEM119 (Transmembrane Protein 119, also known as Osteoblast Induction Factor or OBIF), is an approximately 38-kDa type 1 transmembrane protein that is predominantly expressed in osteoblasts and is upregulated during osteoblastic differentiation (1, 2). TMEM119 is also expressed in a cell line of microglia, and TMEM119 immunoreactivity is observed in a specific subset of microglia in brains of neurodegenerative diseases, such as Alzheimers disease (3). Mature human TMEM119 consists of a 71 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 166 aa cytoplasmic domain. Within the ECD, human TMEM119 shares 78% and 75% aa sequence identity with mouse and rat TMEM119, respectively. TMEM-119 is involved in the osteoblast differentiation and bone development by acting as a ligand and has been reported to contribute to the proliferation, migration, and invasion of osteosarcoma cells, as well as functioning as an oncogene in osteosarcoma (3, 4).
References
- Jiang, Z,H. et al. (2017) Expt & Mol Med. 49:e329.
- Mizuhashi, K. et al. (2012) Dev. Growth Differ. 54:474.
- Satoh, J. et al. (2016) Neuropathol. 36:39.
- Kanamoto, T. et al. (2009) BMC Develop. Biol. 9:70.
Long Name
Transmembrane Protein 119
Alternate Names
OBIF
Gene Symbol
TMEM119
UniProt
Additional TMEM119 Products
Product Documents for Human TMEM119 Antibody
Product Specific Notices for Human TMEM119 Antibody
For research use only
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