HEK293 human embryonic kidney cell line transfected with (A) human TMPRSS2 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human TMPRSS2 Monoclonal Antibody (Catalog # MAB107231) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB0041). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human TMPRSS2 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human TMPRSS2 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMPRSS2

TMPRSS2 belongs to the serine protease family. It contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. TMPRSS2 facilitates human SARS coronavirus (SARS-CoV) infection via two independent mechanisms: proteolytic cleavage of ACE2, which might promote viral uptake, and cleavage of coronavirus spike glycoprotein, which activates the glycoprotein for cathepsin Lindependent host cell entry. Alternatively spliced transcripts encoding different proteins have been described.

Long Name

Transmembrane protease, serine 2

Alternate Names

Epitheliasin, PP9284, PRSS10

Entrez Gene IDs

7113 (Human); 50528 (Mouse); 102137447 (Cynomolgus Monkey)

Gene Symbol

TMPRSS2

UniProt

O15393 (Human)

Additional TMPRSS2 Products

Product Documents for Human TMPRSS2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TMPRSS2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human TMPRSS2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB107231

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all TMPRSS2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human TMPRSS2 Antibody

Detection of TMPRSS2 in HEK293 Human Cell Line Transfected with Human TMPRSS2 and eGFP by Flow Cytometry.