HEK293 human embryonic kidney cell line transfected with (A) human TMPRSS2 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human TMPRSS2 Monoclonal Antibody (Catalog # MAB10723) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB0041). Staining was performed using our Staining Membrane-associated Proteins protocol.

TMPRSS2 in LNCaP Human Cell Line.

TMPRSS2 was detected in immersion fixed LNCaP human prostate cancer cell line (positive staining) and A549 human lung carcinoma cell line (negative staining) using Mouse Anti-Human TMPRSS2 Monoclonal Antibody (Catalog # MAB10723) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human TMPRSS2 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human TMPRSS2 and eGFP

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed LNCaP human prostate cancer cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMPRSS2

TMPRSS2 is a transmembrane serine protease. The TMPRSS2 gene is upregulated by androgenic hormones in prostate cells and downregulated in androgen-independent prostate cancer. The fusion between TMPRSS2 gene and ERG is the most common chromosomal aberration detected in prostate cancer. In relation to COVID-19, TMPRSS2 is involved in priming the viral spike protein which facilitates viral entry. TMPRSS2 inhibitors are candidates for treatment options of SARS-CoV-2 and other coronaviruses.

Long Name

Transmembrane protease, serine 2

Alternate Names

Epitheliasin, PP9284, PRSS10

Entrez Gene IDs

7113 (Human); 50528 (Mouse); 102137447 (Cynomolgus Monkey)

Gene Symbol

TMPRSS2

UniProt

O15393 (Human)

Additional TMPRSS2 Products

Product Documents for Human TMPRSS2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TMPRSS2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human TMPRSS2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10723

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all TMPRSS2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human TMPRSS2 Antibody

Detection of TMPRSS2 in HEK293 Human Cell Line Transfected with Human TMPRSS2 and eGFP by Flow Cytometry