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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 22221

Product Specifications

Immunogen

E. coli-derived recombinant human TNF RIIExtracellular domain

Specificity

Detects human TNF RII in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) 4‑1BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLymophotactin beta R, rhNGF R, rhOPG, recombinant mouse (rm) OX40, rhRANK, rhTAJ, rhTNF RI, or rmTNF RII.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human TNF RII/TNFRSF1B PE-conjugated Antibody

Detection of Human TNFRII in Human whole blood granulocytes by Flow Cytometry.

Detection of Human TNFRII in Human whole blood granulocytes by Flow Cytometry.

Human whole blood granulocytes were stained with Mouse Anti-Human TNFRII Phycoerythrin Monoclonal Antibody (FAB11226P, filled histogram) or Mouse IgG2A Isotype Control (IC003P, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

Detection of TNF RII/TNFRSF1B in U937 cells by Flow Cytometry.

U937 cells were stained with Mouse Anti-Human TNF RII/TNFRSF1B PE-conjugated Monoclonal Antibody (Catalog # FAB11262P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TNF RII/TNFRSF1B PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood granulocytes and U937 human histiocytic lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TNF RII/TNFRSF1B

TNF RII is a type I transmembrane glycoprotein primarily expressed on hematopoietic cells and is designated TNFRSF1B. TNF RII is a high-affinity receptor for TNF‑ alpha and TNF-beta. A soluble form of the receptor generated by proteolytic cleavage functions as a TNF antagonist.

Long Name

Tumor Necrosis Factor Receptor II

Alternate Names

CD120b, TNFRII, TNFRSF1B

Entrez Gene IDs

7133 (Human); 21938 (Mouse); 156767 (Rat); 102144224 (Cynomolgus Monkey)

Gene Symbol

TNFRSF1B

Additional TNF RII/TNFRSF1B Products

Product Documents for Human TNF RII/TNFRSF1B PE-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TNF RII/TNFRSF1B PE-conjugated Antibody

For research use only

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