Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4770
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Canine
Applications
Validated:
CyTOF-reported, Flow Cytometry, Immunocytochemistry
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Labeling, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Clone # TRA-1-60
Product Specifications
Immunogen
2102Ep human embryonal carcinoma cell line
Specificity
Detects human TRA‑1‑60(R).
Clonality
Monoclonal
Host
Mouse
Isotype
IgM
Scientific Data Images for Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody
Detection of TRA‑1‑60 in BG01V Human Cells by Flow Cytometry
BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-60 Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770, filled histogram) or isotype control antibody followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (F0116).
TRA‑1‑60 in BG01V Human Stem Cells.
TRA-1-60 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-60 Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (yellow; NL019) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
TRA‑1‑60(R) in ADLF1 and FAB2 Stem Cell Lines.
TRA-1-60(R) was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Mouse Anti-Human TRA-1-60(R) Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # MAB4770) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Applications for Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody
Application
Recommended Usage
CyTOF-reported
This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: BG01V human embryonic stem cells and iPSC cells
Sample: BG01V human embryonic stem cells and iPSC cells
Immunocytochemistry
8-25 µg/mL
Sample: Immersion-fixed BG01V human embryonic stem cells immersion fixed ADLF1 and FAB2 induced pluripotent stem cell lines
Sample: Immersion-fixed BG01V human embryonic stem cells immersion fixed ADLF1 and FAB2 induced pluripotent stem cell lines
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB4770 in the following applications:
Formulation, Preparation, and Storage
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRA-1-60(R)
TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells (1). The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors (2‑4). Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan (5). Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope (6).
References
- Andrews, P. et al. (1984) Hybridoma 3:347.
- Thomson, J. et al. (1998) Science 282:1145.
- Giwercman, A. et al. (1993) Cancer 72:1308.
- Marrink, J. et al. (1991) Int. J. Cancer 49:368.
- Badcock, G. et al. (1999) Cancer Res. 59:4715.
- Schopperle, W. and W. DeWolf (2007) Stem Cells 25:723.
Long Name
Tumor-related Antigen-1-60 [R]
Alternate Names
TRA160(R)
Entrez Gene IDs
5420 (Human)
Gene Symbol
PODXL
Additional TRA-1-60(R) Products
Product Documents for Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody
Product Specific Notices for Human TRA-1-60(R) Neuraminidase Resistant Epitope Antibody
For research use only
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