Detects human TRAIL R3/TNFRSF10C in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) TRAIL R2, rhTRAIL R1, or rhTRAIL R4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human TRAIL R3/TNFRSF10C APC-conjugated Antibody

Detection of TRAIL R3/TNFRSF10C in Human PBMC Monocytes by Flow Cytometry.

Human peripheral blood mononuclear cell (PBMC) Monocytes were stained with Mouse Anti-Human TRAIL R3/TNFRSF10C APC-conjugated Monoclonal Antibody (Catalog # FAB6302A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of TRAIL R3/TNFRSF10C in Human Blood Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with Mouse Anti-Human TRAIL R3/TNFRSF10C APC-conjugated Monoclonal Antibody (Catalog # FAB6302A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TRAIL R3/TNFRSF10C APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample:

Human peripheral blood mononuclear cell (PBMC) Monocytes and human peripheral blood granulocytes

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRAIL R3/TNFRSF10C

Human TRAIL R3, also known as DcR1 (Decoy Receptor 1), LIT, and TRID, is a glycosyl-phosphatidylinositol-linked membrane protein which binds TRAIL (Apo2 Ligand) with high affinity. In the new TNF superfamily nomenclature, TRAIL R3 is referred to as TNFRSF10C. TRAIL R3 has the TRAIL-binding extracellular cysteine-rich domains but lacks the intracellular signalling domain. As a result, binding of TRAIL to TRAIL R3 does not transduce an apoptosis signal. Expression of TRAIL R3 has been shown to protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-induced apoptosis. A second TRAIL decoy receptor, which binds TRAIL with high-affinity but antagonizes TRAIL-induced apoptosis, named TRAIL R4, DcR2 or TRUNDD, has also been reported. The human soluble TRAIL R3/Fc chimera neutralizes the ability of TRAIL to induce apoptosis.

References

Sheridan, J.P. et al. (1997) Science 277:818.
Golstein, P. (1997) Curr. Biol. 7:R750.

Long Name

TRAIL Receptor 3

Alternate Names

CD263, DcR1, TNFRSF10C, TRAILR3

Entrez Gene IDs

8794 (Human)

Gene Symbol

TNFRSF10C

UniProt

O14798

Additional TRAIL R3/TNFRSF10C Products

Product Documents for Human TRAIL R3/TNFRSF10C APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human TRAIL R3/TNFRSF10C APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human TRAIL R3/TNFRSF10C APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6302A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications