Detects recombinant human TRAIL R1/TNFRSF10A in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TRAIL R2, rhTRAIL R3, rhTRAIL R4, or rhDcR3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human TRAILR1/TNFRSF10A APC-conjugated Antibody

Detection of TRAIL R1/TNFRSF10A in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human TRAIL R1/TNFRSF10A APC-conjugated Monoclonal Antibody (Catalog # FAB347A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TRAILR1/TNFRSF10A APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: HeLa human cervical epithelial carcinoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using FAB347A in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

For liquid material, refer to CoA for concentration.

Formulation

Supplied in a saline solution containing BSA and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRAILR1/TNFRSF10A

Human TRAIL R1, also called DR4, is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand). In the TNF superfamily nomenclature, TRAIL R1 is referred to as TNFRSF10A. TRAIL R1 cDNA encodes a 468 amino acid precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among the TNF receptor family proteins, TRAIL R1 is most closely related to TRAIL R2/DR5, sharing 55% amino acid sequence identity. Binding of trimeric TRAIL to TRAIL R1 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R1/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R1, an additional TRAIL R2/DR5, which tranduces apoptosis signal, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.

References

Pan, G. et al. (1997) Science 276:111.
Golstein, P. (1997) Curr. Biol. 7:R750.

Long Name

TRAIL Receptor 1

Alternate Names

CD261, DR4, TNFRSF10A, TRAIL R1

Entrez Gene IDs

8797 (Human)

Gene Symbol

TNFRSF10A

UniProt

AAC51226

Additional TRAILR1/TNFRSF10A Products

Product Documents for Human TRAILR1/TNFRSF10A APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human TRAILR1/TNFRSF10A APC-conjugated Antibody

For research use only

Citations
Reviews

Calculators
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Flow Cytometry

Human TRAILR1/TNFRSF10A APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB347A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications