Human peripheral blood mononuclear cells (PBMCs) treated with PHA were stained with Mouse Anti-Human IL-2 Ra APC-conjugated Monoclonal Antibody (Catalog # FAB1020A) and either (A) Mouse Anti-Human TRANCE/TNFSF11/RANK L PE-conjugated Monoclonal Antibody (Catalog # FAB6264P) or (B) Mouse IgG_2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Detection of TRANCE/TNFSF11/RANK L in HEK293 human cell line transfected with human TRANCE by Flow Cytometry.

Human HEK293 cell line transfected with human TRANCE was stained with Mouse Anti-Human TRANCE/TNFSF11/RANK L PE-conjugated Monoclonal Antibody (Catalog # FAB6264P, filled histogram) or Mouse IgG2B PE Isotype Control(Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TRANCE/TNFSF11/RANK L PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PHA or HEK293 human cell line transfected with human TRANCE

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRANCE/TNFSF11/RANK L

TNF-related activation-induced cytokine (TRANCE; also RANK L, OPGL, and ODF) is a 35 kDa (predicted) type II transmembrane glycoprotein and member of the TNF cytokine family. Human TRANCE is 317 amino acids (aa) in length and contains a 47 aa cytoplasmic region, a 21 aa transmembrane region, and a 249 extracellular domain (ECD), which contains two potential sites of N-linked glycosylation. Splicing variants produce three isoforms for human TRANCE. Isoform 1 is the standard form. In isoform 2, aa corresponding to 1-73 in isoform 1 are missing. In isoform 3, aa 1-47 are missing. Human TRANCE ECD is 83% identical to mouse TRANCE ECD. TRANCE is expressed highest in the peripheral lymph nodes and weaker in the spleen, peripheral blood leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach, and thyroid. TRANCE plays a role in osteoclast differentiation and activation.

Long Name

TNF-related Activation-induced Cytokine

Alternate Names

CD254, ODF, OPGL, RANK L, RANKL, TNFSF11

Entrez Gene IDs

8600 (Human); 21943 (Mouse); 117516 (Rat); 102137946 (Cynomolgus Monkey)

Gene Symbol

TNFSF11

UniProt

AAC51762

Additional TRANCE/TNFSF11/RANK L Products

Product Documents for Human TRANCE/TNFSF11/RANK L PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TRANCE/TNFSF11/RANK L PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human TRANCE/TNFSF11/RANK L PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6264P

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all TRANCE/TNFSF11/RANK L Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human TRANCE/TNFSF11/RANK L PE-conjugated Antibody

Detection of TRANCE/TNFSF11/RANK L in Human PBMCs by Flow Cytometry.