Human Transferrin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5746

R&D Systems, part of Bio-Techne
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MAB5746
MAB5746-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 507506

View all Transferrin Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transferrin
Val20-Pro698
Accession # AAH59367

Specificity

Detects human Transferrin in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Transferrin Antibody

Detection of Human Transferrin by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line (negative control) and human liver. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Transferrin Monoclonal Antibody (Catalog # MAB5746) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Transferrin at approximately 67-70 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.

Applications for Human Transferrin Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human liver tissue, under non-reducing condtions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Transferrin

Transferrin, also known as serotransferrin and siderophilin, is an 80 kDa secreted glycoprotein that binds two Fe (3+) ions, transporting them from sites of absorption and heme degradation to those of storage and utilization. It is a member of the Transferrin family of proteins. Human Transferrin is synthesized as a 698 amino acid (aa) precursor that contains a 19 aa signal sequence and a 679 aa mature chain. The mature chain consists of two Transferrin-like domains, two high-affinity iron binding sites, one potential site for O-linked glycosylation, and two potential sites for N-linked glycosylation. Human Transferrin is 73% aa identical to mouse and rat Transferrin.

Alternate Names

Beta-1 metal-binding globulin, Serotransferrin, Siderophilin, TF

Entrez Gene IDs

7018 (Human)

Gene Symbol

TF

UniProt

AAH59367

Additional Transferrin Products

Product Documents for Human Transferrin Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Transferrin Antibody

For research use only

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