Human Transglutaminase 3/TGM3 Antibody

Detection of Human and Mouse Transglutaminase 3/TGM3 by Western Blot.

Western blot shows lysates of B16-F1 mouse melanoma cell line, Bowes human melanoma cell line, and human skin tissue. PVDF Membrane was probed with 1 µg/mL of Human Transglutaminase 3/TGM3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4604) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). For additional reference, Recombinant Human Transglutaminase 3/TGM3 (Catalog # 4604-TG) (10 ng/lane) uncleaved and cleaved by recombinant human Cathepsin were included. Specific bands were detected for uncleaved Transglutaminase 3/TGM3 at approximately 77 kDa (as indicated) and Transglutaminase 3/TGM3 cleavage products at approximately 50 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Transglutaminase 3/TGM3 in Human Esophagus.

Transglutaminase 3/TGM3 was detected in immersion fixed paraffin-embedded sections of human esophagus using Human Transglutaminase 3/TGM3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4604) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cells in the papillae layer in mucosa. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.