Human Trefoil Factor 3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4407
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
ELISA Development, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 415909
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TFF3
Ala21-Phe80
Accession # Q07654
Ala21-Phe80
Accession # Q07654
Specificity
Detects human TFF3 in direct ELISAs and Western blots. Does not cross-react with recombinant human TFF2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Trefoil Factor 3 Antibody
TFF3 in Human Colon.
TFF3 was detected in immersion fixed paraffin-embedded sections of human colon using 25 µg/mL Human TFF3 Monoclonal Antibody (Catalog # MAB4407) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of goblet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Trefoil Factor 3 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon
Sample: Immersion fixed paraffin-embedded sections of human colon
Western Blot
1 µg/mL
Sample: Recombinant Human TFF3
under non-reducing conditions only
Sample: Recombinant Human TFF3
under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 5 reviews rated 4.4 using MAB4407 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TFF3
Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes. Trefoils contribute to protection and repair of the gastrointestinal tract. TFF3 is secreted by mucus-producing goblet cells in the large and small intestines and lower respiratory tract. It is mainly active as a disulfide-linked homodimer. The mature 59 aa, 6.5 kDa human TFF3 contains one trefoil structure formed by intramolecular disulfides and shows 76% aa identity with mouse TFF3.
Long Name
Trefoil Factor 3
Alternate Names
ITF, TFI
Gene Symbol
TFF3
UniProt
Additional TFF3 Products
Product Documents for Human Trefoil Factor 3 Antibody
Product Specific Notices for Human Trefoil Factor 3 Antibody
For research use only
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