Human peripheral blood granulocytes were stained with Mouse Anti-Human TREM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB1278P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of TREM‑1 in THP-1 Human Cell Line by Flow Cytometry

THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human TREM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB1278P, filled histogram) or isotype control antibody (IC002P, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

TREM-1 Specificity is Shown by Flow Cytometry in Knockout Cell Line.

TREM-1 knockout THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human TREM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB1278P, filled histogram) or isotype control antibody (IC002P,open histogram). No staining in the TREM-1 knockout THP-1 cell line was observed. Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human TREM-1 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood granulocytes

Knockout Validated

TREM‑1 is specifically detected in THP-1 human acute monocytic leukemia parental cell line but is not detectable in TREM‑1 knockout THP-1 cell line.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using FAB1278P in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. The natural ligand for TREM-1 has not been identified. However, engagement of TREM-1 with an agonist monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha, suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock. Human and mouse TREM-1 share approximately 42% amino acid sequence homology (1-3).

References

Bouchon, A. (2000) J. Immunol. 164:4991.
Bouchon, A. (2001) Nature 410:1103.
Nathan, C. and A. Ding (2001) Nature Med. 7:530.

Long Name

Triggering Receptor Expressed on Myeloid Cells 1

Alternate Names

CD354, TREM1

Entrez Gene IDs

54210 (Human); 58217 (Mouse)

Gene Symbol

TREM1

UniProt

Q9NP99

Additional TREM-1 Products

Product Documents for Human TREM-1 PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human TREM-1 PE-conjugated Antibody

For research use only

Citations
Reviews

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Flow Cytometry

Human TREM-1 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1278P

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human TREM-1 PE-conjugated Antibody

Detection of TREM‑1 in Human Blood Granulocytes by Flow Cytometry.