Human TREM2 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1828

R&D Systems, part of Bio-Techne
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BAF1828
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry, Western Blot

Cited:

ELISA Capture, ELISA Detection, ELISA Development, ELISA Development (Capture), ELISA Development (Detection), Functional Assay, Immunoprecipitation

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all TREM2 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TREM2
His19-Ser174
Accession # Q9NZC2

Specificity

Detects human TREM2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) TREM2b, rhTREM1, and recombinant mouse TREM3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TREM2 Biotinylated Antibody

TREM2 antibody in Human PBMCs by Immunocytochemistry (ICC).

TREM2 in Human PBMCs.

TREM2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS using Goat Anti-Human TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1828) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of TREM-2 in THP-1 Human Cell Line by Flow Cytometry.

THP-1 human monocytic leukemia cell line was stained with Goat Anti-Human TREM-2 Biotinylated Polyclonal Antibody (Catalog # BAF1828, filled histogram) or control antibody (BAF108, open histogram), followed by Phycoerythrin-conjugated Streptavidin (F0040). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human TREM2 Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS

Western Blot

0.1 µg/mL
Sample: Recombinant Human TREM2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREM2

TREM2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa molecular weight type I transmembrane member of the TREM family and Ig superfamily . Mature human TREM2 consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. Within the ECD, human TREM2 shares 73% and 74% aa sequence identity with mouse and rat TREM2, respectively. Two closely related transcripts were reported in mouse and designated TREM2a and TREM2b. Soluble forms of the TREM2 ECD are generated by alternative splicing or proteolytic cleavage, and the cytoplasmic domain can be liberated by gamma-Secretase mediated intramembrane cleavage. It is a pattern recognition receptor that binds anionic ligands. A positively charged lysine within the transmembrane segment allows association with the signal adapter protein, DAP12 to deliver an activating signal that plays a role in both innate and adaptive immune responses, including inhibition of macrophage activation. TREM2 is expressed on macrophages, immature myeloid dendritic cells, osteoclasts, microglia, and adipocytes. It promotes the differentiation and function of osteoclasts, the production of inflammatory cytokines by adipocytes, insulin resistance, and the phagocytic clearance of bacteria. In the CNS, TREM2 binds to ApoE, ApoA1, and ApoB and mediates the clearance of apoptotic neurons, amyloid plaques, and cell debris following demyelination. TREM2 also interacts with and modifies signaling through Plexin A1 on dendritic cells and osteoclasts. Mutations in TREM2 or DAP12 are associated with the development of Alzheimer's disease and Nasu-Hakola disease (NHD/PLOSL) which is characterized by presenile dementia and bone cysts. Soluble TREM2 is elevated in cerebrospinal fluid of patients with active multiple sclerosis (MS), and TREM2 blockade exacerbates disease symptoms in the experimental EAE model of MS.

Long Name

Triggering Receptor Expressed on Myeloid Cells 2

Alternate Names

PLOSL2, TREM-2

Entrez Gene IDs

54209 (Human); 102133279 (Cynomolgus Monkey)

Gene Symbol

TREM2

UniProt

Q9NZC2

Additional TREM2 Products

Product Documents for Human TREM2 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human TREM2 Biotinylated Antibody

For research use only

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