Human TRIF/TICAM1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6216
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Simple Western, Western Blot
Cited:
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human TRIF/TICAM1
Lys29-Ala204
Accession # Q8IUC6
Lys29-Ala204
Accession # Q8IUC6
Specificity
Detects human TRIF/TICAM1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human TRIF/TICAM1 Antibody
Detection of Human TRIF/TICAM1 by Western Blot.
Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.25 µg/mL of Human TRIF/TICAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6216) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRIF/TICAM1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
TRIF/TICAM1 in Raji Human Cell Line.
TRIF/TICAM1 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Human TRIF/TICAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6216) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Human TRIF/TICAM1 by Simple WesternTM.
Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for TRIF/TICAM1 at approximately 122 kDa (as indicated) using 25 µg/mL of Goat Anti-Human TRIF/TICAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6216) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human TRIF/TICAM1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed Raji human Burkitt's lymphoma cell line
Sample: Immersion fixed Raji human Burkitt's lymphoma cell line
Simple Western
25 µg/mL
Sample: Raji human Burkitt's lymphoma cell line
Sample: Raji human Burkitt's lymphoma cell line
Western Blot
0.25 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line
Sample: Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRIF/TICAM1
TRIF (TIR domain-containing adaptor inducing IFN-beta ; also TICAM1) is a 105-110 kDa cytoplasmic adaptor molecule that mediates Toll receptor signaling. It is widely expressed and associates with both TLR3 and TLR4. Relative to TLR3, TRIF appears to activate IRF 3, -4, and -7, as well as NF kappaB and FADD. Its action on FADD is through RIP1, and this induces apoptosis. Human TRIF is 712 amino acids (aa) in length and contains three TRAF6 bonding motifs (aa 84-91, 248-255 and 299-309), one TIR domain (aa 390-460), a Pro-rich region (aa 614-678), and an overlapping RHIM domain (aa 661-699). The molecule is reported to form a homodimer. There are multiple potential isoform variants. One shows a 23 aa substitution for aa 31-162 accompanied by a Pro substitution for aa 633-660, a second shows a 44 aa substitution for aa 352-712, and a third shows a 38 aa substitution for aa 271-712. Over aa 29-204, human TRIF shares 62% aa identity with mouse TRIF.
Long Name
Toll-like receptor adaptor molecule 1
Alternate Names
TICAM1
Gene Symbol
TICAM1
UniProt
Additional TRIF/TICAM1 Products
Product Documents for Human TRIF/TICAM1 Antibody
Product Specific Notices for Human TRIF/TICAM1 Antibody
For research use only
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