Human Troponin I/TNNI3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB85941

R&D Systems, part of Bio-Techne
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MAB85941-100
MAB85941-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Rat

Applications

Validated:

ELISA

Cited:

ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 923945

View all Troponin I/TNNI3 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Troponin I/TNNI3
Met1-Ser210
Accession # P19429

Specificity

Detects human Troponin I/TNNI3 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Troponin I/TNNI3 Antibody

Human Troponin I Antibody in ELISA Standard Curve.

Human Troponin I/TNNI3 ELISA Standard Curve.

Recombinant Human Troponin I/TNNI3 protein was serially diluted 2-fold and captured by Mouse Anti-Human Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB85942) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB85941) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human Troponin I/TNNI3 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB85942).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Troponin I/TNNI3

Troponin I, also known as TNI, is a 24-29 kDa component of a protein complex on striated muscle thin filaments. Troponin I inhibits the calcium-dependent muscle contraction mediated by Troponins C and T. The expression of cardiac Troponin I (TNNI3) is restricted to cardiac muscle, while TNNI1 and TNNI2 (encoded by distinct genes) are expressed in skeletal muscle. Mutations of cardiac Troponin I are associated with heriditary cardiomyopathy. Human cardiac Troponin I shares 93% amino acid sequence identity with mouse and rat cardiac Troponin I.

Long Name

Troponin I3, Cardiac Type

Alternate Names

CMD2A, CTnI, RCM1, TNNC1, TNNI3,

Entrez Gene IDs

7137 (Human)

Gene Symbol

TNNI3

UniProt

P19429

Additional Troponin I/TNNI3 Products

Product Documents for Human Troponin I/TNNI3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Troponin I/TNNI3 Antibody

For research use only

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