Human Trypsin 3/PRSS3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB37101

R&D Systems, part of Bio-Techne
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MAB37101
MAB37101-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 420012

View all Trypsin 3/PRSS3 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Trypsin 3/PRSS3
Val16-Ser247
Accession # NP_002762

Specificity

Detects human Trypsin 3/PRSS3 in direct ELISAs and Western blots. Detects both pro and mature forms of Trypsin 3 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Trypsin 1, 2, recombinant mouse Trypsin 3, or Chymostrypsin is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human Trypsin 3/PRSS3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Trypsin 3/PRSS3 (Catalog # 3710-SE)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Trypsin 3/PRSS3

Human Trypsin 3, encoded by the PRSS3 gene, is also known as mesotrypsin (1). Constituting less than 10% of the total trypsin content in normal pancreatic juice, it is one of the three trypsin isoforms produced by the pancreas (2). Compared to Trypsin 1 and 2, one intriguing feature of Trypsin 3 is its resistance to polypeptide trypsin inhibitors, such as the Kunitz-type soybean trypsin inhibitor or the Kazal-type pancreatic secretory trypsin inhibitor. As revealed by the crystal structure, this resistance is likely due to the presence of an arginine residue in place of the highly conserved Gly198 (3). Trypsin 3 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. One physiologic function of Trypsin 3 has been proposed to be degradation of trypsin inhibitors, which facilitates the digestion of those foods rich in these proteins (4).The amino acid sequence of human Trypsin 3 shares 76%, 74%, 72% and 67% identity with that of rat, mouse, dog and chicken, respectively.

References

  1. Nyaruhucha, C.N.M. et al. (1997) J. Biol. Chem. 272:10573.
  2. Rinderknecht, H. et al. (1984) Gastroenterology 86:681.
  3. Katona, G. et al. (2002) J. Mol. Biol. 315:1209.
  4. Szmola, R. et al. (2003) J. Biol. Chem. 278:48580.

Alternate Names

Mesotrypsin, MTG, PRSS3, PRSS4, Trypsin 4, Trypsinogen 15

Entrez Gene IDs

5646 (Human); 22073 (Mouse); 362347 (Rat)

Gene Symbol

PRSS3

UniProt

NP_002762

Additional Trypsin 3/PRSS3 Products

Product Documents for Human Trypsin 3/PRSS3 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Trypsin 3/PRSS3 Antibody

For research use only

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