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Human Tryptase gamma-1/TPSG1 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1667

R&D Systems, part of Bio-Techne
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BAF1667

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Tryptase gamma‑1/TPSG1
Arg20-Arg281
Accession # Q9NRR2

Specificity

Detects human Tryptase gamma‑1/TPSG1 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) Tryptase alpha-1 and rhTryptase beta-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Tryptase gamma-1/TPSG1 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Tryptase  gamma-1/TPSG1 (Catalog # 1667-SE)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tryptase gamma-1/TPSG1

As mediators of inflammatory and allergic response, mast cells are found throughout the body concentrated near blood vessels in connective tissue and the mucous membranes of the respiratory and gastrointestinal tract (3). Upon activation, human mast cells release granules that are enriched with neutral serine proteases including Tryptases, chymase and cathepsin G (4). Tryptase gamma-1, also called transmembrane Tryptase, is encoded by TPSG1, one of many serine protease genes clustered in human chromosome 16p13.3 (5). Human Tryptase gamma-1 is synthesized as a 321 amino acid preproenzyme with a C-terminal transmembrane anchor (1, 2). The rhTPSG1 was expressed as a soluble protein terminated at residue 281 and corresponded to the proenzyme. The proenzyme can be cleaved by trypsin to form the active enzyme. The serine protease activity of trypsin-activated rhTPSG1 can be inhibited by ecotin (R&D Systems, Catalog # 1328-PI). Greater than 95% protease activity is inhibited by ecotin at approximately 10:1 molar ratio.

References

  1. Wong, G.W. et al. (1999) J. Biol. Chem. 274:30784.
  2. Caughey, G.H. et al. (2000) J. Immunol. 164:6566.
  3. Harris, J.L. et al. (2001) J. Biol. Chem. 276:34941.
  4. Miller, H.R.P. and A.D. Pemberton (2002) Immunology 105:375.
  5. Wong, G.W. et al. (2002) J. Biol. Chem. 277:41906.

Alternate Names

TPSG1, Tryptase gamma1

Entrez Gene IDs

25823 (Human)

Gene Symbol

TPSG1

UniProt

Additional Tryptase gamma-1/TPSG1 Products

Product Documents for Human Tryptase gamma-1/TPSG1 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Tryptase gamma-1/TPSG1 Biotinylated Antibody

For research use only

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