Human Tryptophan Hydroxylase 1/TPH-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5276
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 508417
Product Summary for Human Tryptophan Hydroxylase 1/TPH-1 Antibody
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Tryptophan Hydroxylase 1/TPH‑1
Met102-Pro403
Accession # P17752
Met102-Pro403
Accession # P17752
Specificity
Detects human Tryptophan Hydroxylase 1/TPH‑1 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human Tryptophan Hydroxylase 1/TPH-1 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Tryptophan Hydroxylase 1/TPH‑1, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Tryptophan Hydroxylase 1/TPH‑1, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Tryptophan Hydroxylase 1/TPH‑1
Sample: Recombinant Human Tryptophan Hydroxylase 1/TPH‑1
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tryptophan Hydroxylase 1/TPH-1
Tryptophan Hydroxylase-1 (TPH-1) is a member of the pterin-dependent aromatic acid hydroxylase family. It catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. The amino acid sequence of human TPH-1 is 96%, 90%, and 87% identical to that of cow, mouse, and chicken.
Alternate Names
TPH-1, TPRH
Gene Symbol
TPH1
UniProt
Additional Tryptophan Hydroxylase 1/TPH-1 Products
Product Documents for Human Tryptophan Hydroxylase 1/TPH-1 Antibody
Product Specific Notices for Human Tryptophan Hydroxylase 1/TPH-1 Antibody
For research use only
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