NS0 mouse myeloma cell line co-transfected with human TSPAN2 and eGFP was stained with either (A) Mouse IgG_2BIsotype Control (Catalog # MAB0041) or (B) Mouse Anti-Human TSPAN2 Monoclonal Antibody (Catalog # MAB7876) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human TSPAN2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human TSPAN2 and eGFP co-transfectants

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSPAN2

TSPAN2 (tetraspanin-2) is a member of the transmembrane 4 superfamily. It is a cell surface 221 amino acid (aa) protein with 4 transmembrane segments and a large extracellular loop (aa 112‑188) that contains four conserved cysteines and one potential N-glycosylation site. Both the N- and C-termini are intracellular. In humans, TSPAN2 expressed sequence tags have been found in the pregnant uterus, T cell, and fetal heart. In rats, TSPAN2 is thought to be involved in oligodendrocyte differentiation. Human TSPAN2 shares 94% and 95% aa sequence identity with mouse and rat TSPAN2, respectively.

Long Name

Tetraspanin 2

Alternate Names

NET3, TSN2

Entrez Gene IDs

10100 (Human); 70747 (Mouse); 64521 (Rat)

Gene Symbol

TSPAN2

UniProt

O60636

Additional TSPAN2 Products

Product Documents for Human TSPAN2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TSPAN2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human TSPAN2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7876

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all TSPAN2 Antibodies »

Product Summary for Human TSPAN2 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human TSPAN2 Antibody

Detection of TSPAN2 in NS0 Mouse Cell Line Co-transfected with TSPAN2 and eGFP by Flow Cytometry.