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Human TSPAN8 Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB4734G

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 458811

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human TSPAN8
Met1-Lys237 (Ile35Val)
Accession # AAH05246

Specificity

Detects human TSPAN8 in Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Human TSPAN8 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: HT-29 human colon adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TSPAN8

Tetraspanin-8 (TSPAN8), also known as TM4SF3 and human tumor-associated antigen CO-0029, is a member of the transmembrane 4 superfamily. It is a cell surface 27‑34 kDa glycoprotein with 4 transmembrane segments, and a large extracellular loop (LEL) domain from amino acids (aa) 106‑206. Both the N- and C-termini are intracellular. TSPAN8 is expressed by multiple tumor types as well as smooth and skeletal muscle cells, endothelial cells, hematopoietic progenitor cells and non-keratinized squamous epithelium. As with other TSPAN family molecules, TSPAN8 acts as an organizer of microdomains in membranes. Molecules that may be found in these domains include CD151 (another TSPAN family member), beta1 Integrins, Integrin alpha4 beta6, EpCAM, and CD13. TSPAN8 would appear to remove Integrins from the cell surface, facilitating cell motility which, in the case of tumor cells, results in metastasis. TSPAN8 shares 53% aa sequence identity with both mouse and rat TSPAN8.

Long Name

Tetraspanin 8/Transmembrane 4 Superfamily, Member 3

Alternate Names

CO-029, TM4SF3

Entrez Gene IDs

7103 (Human); 216350 (Mouse); 171048 (Rat)

Gene Symbol

TSPAN8

UniProt

Additional TSPAN8 Products

Product Documents for Human TSPAN8 Alexa Fluor® 488-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TSPAN8 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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