Human Twist-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6249
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Twist-2
Met1-Ser159
Accession # Q8WVJ9
Met1-Ser159
Accession # Q8WVJ9
Specificity
Detects human Twist-2 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human Twist-1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Twist-2 Antibody
Twist‑2 in A549 Human Cell Line.
Twist-2 was detected in immersion fixed A549 human lung carcinoma cells treated with Recombinant Human TGF-beta 1 (Catalog # 240-B) using Human Twist-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6249) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Twist-2 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line treated with Recombinant Human TGF-beta 1 (Catalog # 240-B)
Sample: Immersion fixed A549 human lung carcinoma cell line treated with Recombinant Human TGF-beta 1 (Catalog # 240-B)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Twist-2
Twist-2 (Twist-related protein 2; also Dermo-1 and bHLHa39) is a 19-20 kDa Class B member of the bHLH transcription factor family of proteins. It is expressed in embryonic mesodermal and ectodermal derivatives, and is widely expressed in adult cells, including preosteoblasts, gastric chief cells, preadipocytes and myeloid progenitor cells. Twist-2 is a transcriptional repressor that heterodimerizes with multiple partners such as E12, SREBP1 and MEF2. Human Twist-2 is 160 amino acids (aa) in length. It contains a bHLH domain (aa 65-122) with an embedded DNA-binding motif. Full length human Twist-2 shares 82% and 99% aa identity with human Twist-1 and mouse Twist-2, respectively.
Long Name
Twist Related Protein 2 [Drosophila]
Alternate Names
bHLHa39, DERMO1, Twist2
Entrez Gene IDs
117581 (Human)
Gene Symbol
TWIST2
UniProt
Additional Twist-2 Products
Product Documents for Human Twist-2 Antibody
Product Specific Notices for Human Twist-2 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...