Human Tyrosylprotein Sulfotransferase 2/TPST2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6236
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recominant human Tyrosylprotein Sulfotransferase 2/TPST2
Gln26-Ser377 (predicted)
Accession # O60704
Gln26-Ser377 (predicted)
Accession # O60704
Specificity
Detects human Tyrosylprotein Sulfotransferase 2/TPST2 in direct ELISAs and Western blots. In direct ELISAs, less than 7% cross-reactivity with recombinant human TPST1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Tyrosylprotein Sulfotransferase 2/TPST2 Antibody
Detection of Human Tyrosylprotein Sulfotransferase 2/TPST2 by Western Blot.
Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Tyrosylprotein Sulfotransferase 2/TPST2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6236) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tyrosylprotein Sulfotransferase 2/TPST2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Tyrosylprotein Sulfotransferase 2/TPST2 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: Human pancreas tissue
Sample: Human pancreas tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tyrosylprotein Sulfotransferase 2/TPST2
Human Tyrosylprotein Sulfotransferase 2 (TPST2) is a 42 kDa (predicted) member of a sulfotransferase family. Two human tyrosylprotein sulfotransferases (TPSTs), TPST1 and TPST2 mediate tyrosine O-sulfation of more than 60 different proteins in the Golgi, transferring sulfate from 3'-phosphoadenosine 5'‑phosphosulfate to tyrosine residues contained in polypeptides with acidic motifs to form a tyrosine O-sulfate ester. TSPT1 and TSPT2 are widely expressed, they show similar tissue distribution and overlapping substrate specificity.
Alternate Names
Tyrosylprotein Sulfotransferase 2
Entrez Gene IDs
8459 (Human)
Gene Symbol
TPST2
UniProt
Additional Tyrosylprotein Sulfotransferase 2/TPST2 Products
Product Documents for Human Tyrosylprotein Sulfotransferase 2/TPST2 Antibody
Product Specific Notices for Human Tyrosylprotein Sulfotransferase 2/TPST2 Antibody
For research use only
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