Human Uromodulin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5144
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 877914
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Uromodulin
Asp25-Ser614
Accession # P07911
Asp25-Ser614
Accession # P07911
Specificity
Detects human Uromodulin in ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Uromodulin Antibody
Detection of Human Uromodulin by Western Blot.
Western blot shows lysates of human kidney (medulla) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Uromodulin Monoclonal Antibody (Catalog # MAB5144) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Uromodulin at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions using Immunoblot Buffer Group 1.
Uromodulin in Human Ovary.
Uromodulin was detected in formalin fixed paraffin-embedded sections of human ovary tissue using Mouse Anti-Human Uromodulin Monoclonal Antibody (Catalog # MAB5144) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Uromodulin Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovary
Sample: Immersion fixed paraffin-embedded sections of human ovary
Western Blot
2 µg/mL
Sample: Human kidney (medulla) tissue
Sample: Human kidney (medulla) tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Uromodulin
Uromodulin (also Tamm-Horsfall glycoprotein or THP) is an 85‑95 kDa urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL‑1, TNF-alpha and C1q, activates resting monocytes and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidnay tubules. Human Uromodulin is GPI‑linked. Its proprecursor is 616 amino acids (aa) in length. It contains three EGF-like domains (aa 28‑149), a ZP domain that mediates oligomerization (aa 334‑589) and a cleavable C‑terminal propeptide (aa 615‑640). There are multiple splice variants. One shows a deletion of aa 67‑199, a second shows a nine aa substitution for aa 609‑640, a third shows a Pro substitution for aa 205‑234 and a fourth shows a 66 aa substitution for aa 613‑640. Over aa 25‑614, human Uromodulin is 78% aa identical to mouse Uromodulin.
Alternate Names
ADMCKD2, FJHN, HNFJ, MCKD2, THGP, THP, UMOD, Uromucoid
Gene Symbol
UMOD
UniProt
Additional Uromodulin Products
Product Documents for Human Uromodulin Antibody
Product Specific Notices for Human Uromodulin Antibody
For research use only
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