Human UTF1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3958
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human UTF1
Ser44-Lys153
Accession # Q5T230
Ser44-Lys153
Accession # Q5T230
Specificity
Detects human UTF1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human UTF1 Antibody
UTF1 in BG01V Human Embryonic Stem Cells.
UTF1 was detected in immersion fixed BG01V human embryonic stem cells using Goat Anti-Human UTF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3958) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001). SSEA4 was also detected using Mouse Anti-Human SSEA4 Monoclonal Antibody (Catalog # MAB1435) and stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Cells were counterstained with DAPI (blue). Specific staining of UTF1 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.Applications for Human UTF1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed D3 mouse embryonic stem cell line and BG01V human embryonic stem cells
Sample: Immersion fixed D3 mouse embryonic stem cell line and BG01V human embryonic stem cells
Western Blot
0.1 µg/mL
Sample: Recombinant Human UTF1
Sample: Recombinant Human UTF1
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: UTF1
UTF1 (undifferentiated embryonic cell transcription factor 1) is a 47‑57 kDa transcriptional coactivator. It is expressed mainly in embryonic stem cells, is regulated by Oct-3/4, and promotes rapid cell proliferation. Human UTF1 is a 341 amino acid (aa) phosphoprotein. It is rich in Proline and Arginine residues and contains a
C‑terminal Leu-zipper (aa 286‑313) that is known to interact with the metal-binding motif of ATF-2. Unlike mouse UTF1 which contains an alternate start site at Met43, human UTF1 lacks this Methionine and shows only one isoform. Over amino acids 44‑153, human UTF1 shares 81% aa sequence identity with mouse UTF1.
Long Name
Undifferentiated Embryonic Cell Transcription Factor 1
Alternate Names
undifferentiated embryonic cell transcription factor 1
Gene Symbol
UTF1
UniProt
Additional UTF1 Products
Product Documents for Human UTF1 Antibody
Product Specific Notices for Human UTF1 Antibody
For research use only
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