| Specificity: |
Detects recombinant human VCAM-1 in Direct ELISA.
Applications for Human VCAM-1/CD106 Antibody
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Recommended Concentration
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Sample |
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Immunohistochemistry |
Recommended Concentration
3-25 µg/mL |
Sample
Immersion fixed paraffin-embedded sections of human tonsil |
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Multiplex Immunofluorescence |
Recommended Concentration
15 µg/mL |
Sample
Immersion fixed paraffin-embedded sections of human tonsil |
Scientific Data Examples for Human VCAM-1/CD106 Antibody
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Multiplex Immunofluorescence
Detection of VCAM1 in Human Tonsil via seqIF™ staining on COMET™
VCAM1 was detected in immersion fixed paraffin-embedded sections of human Tonsil using Mouse Anti-Human VCAM1, Monoclonal Antibody (Catalog #MAB11671) at 15ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
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Immunohistochemistry
Detection of VCAM‑1/CD106 in Human Tonsil.
VCAM‑1/CD106 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB11671) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Formulation, Preparation and Storage
| Purification |
Protein A or G purified from cell culture supernatant
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| Reconstitution |
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
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| Formulation |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
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| Shipping |
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
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| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator
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Product Documents for Human VCAM-1/CD106 Antibody
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