Human VCAM-1/CD106 Antibody
R&D Systems, part of Bio-Techne | Catalog # BBA19
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Rabbit
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat Serum
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human VCAM-1
Specificity
Detects VCAM‑1/CD106 in Western blots. VCAM‑1/CD106 has been screened using CHO cells transfected with cDNAs for ICAM-1 and VCAM-1.
Clonality
Polyclonal
Host
Goat
Isotype
Serum
Scientific Data Images for Human VCAM-1/CD106 Antibody
Detection of Human VCAM-1/CD106 by Simple WesternTM.
Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 10 ng/ml Recombinant Human TNF-alpha (210-TA) for 24 hrs, loaded at 0.2 mg/mL. A specific band was detected for VCAM-1/CD106 at approximately 132 kDa (as indicated) using 20 µg/mL of Goat Anti-Human VCAM-1/CD106 Polyclonal Antibody (Catalog # BBA19) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human VCAM-1/CD106 Antibody
Application
Recommended Usage
Immunohistochemistry
1:200 dilution
Sample: Immersion fixed paraffin-embedded sections of human kidney subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human kidney subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Simple Western
20 µg/mL
Sample: HUVEC human umbilical vein endothelial cells
Sample: HUVEC human umbilical vein endothelial cells
Western Blot
1:1000 dilution
Sample: Recombinant Human VCAM-1/CD106 (Catalog # 809-VR)
Sample: Recombinant Human VCAM-1/CD106 (Catalog # 809-VR)
Reviewed Applications
Read 1 review rated 5 using BBA19 in the following applications:
Formulation, Preparation, and Storage
Purification
N/A
Reconstitution
Reconstitute in 0.5 mL of sterile water.
Formulation
Lyophilized from a 0.2 μm filtered solution in Serum.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VCAM-1/CD106
Vascular cell adhesion molecule (VCAM-1) is expressed on the surface of activated endothelial cells and macrophages. It binds to leukocyte integrins VLA-4 and alpha4 beta7 to promote leukocyte adhesion at sites of inflamed vasculature.
Long Name
Vascular Cell Adhesion Molecule 1
Alternate Names
CD106, VCAM1
Gene Symbol
VCAM1
Additional VCAM-1/CD106 Products
Product Documents for Human VCAM-1/CD106 Antibody
Product Specific Notices for Human VCAM-1/CD106 Antibody
For research use only
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